PROJECT 1
RESOMBINANCT DNA TECHNOLGY

• Basic Handling
Handling of equipments properly
• Basic calculations in molecular biology
• Media preparation and autoclaving
• Growth and maintenance of bacteria carrying recombinant plasmid
• Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content
• Restriction analysis
• Gel elution of both vector and insert DNAs Separately
• Ligation reaction
• Competent cells preparation and transformation
• Competent cells efficiency testing using known quantity of DNA
• Screening of transformants through a-complementation test

Probable Duration: 10-12 Days
Fee Amount : Rs. 2,900 only
Option : Polymerase Chain Reaction (PCR) - Machine handling and reaction . Fee amount : Rs. 3,500

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