COURSE 1
RECOMBINANT DNA TECHNOLOGY
- Basic Handling:
- Handling of equipments.
- Basic calculations of molecular biology.
- Media preparation and autoclaving.
- Growth and maintenance of bacteria carrying recombinant plasmid.
- Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content.
- Restriction Digestion and analysis.
- Gel elution of both vector and insert DNA saparately.
- Ligation reaction.
- Competent cell preprtion and transformation.
- Competent cell efficiency testion using known quantity of DNA.
- Screening of trsanformation through α-complementation test.
Probeble Duration : 10-12 WOrking Days
Fee Ammount 4500.00
Option:Polymerase chain reaction (PCR) - machine handling & reaction. Fee ammount : RS 5250.00
COURSE 2
GENETIC ENGINEERING
In addition to all the contents of COURSE-I, this will have the following aspects :
- Screening of transformants by restriction analysis.
- Transferring the gene constructs in binary vectors to Agrobacterium through triparental mating (TPM)
- Transferring of gene to plant system through Agrobacterium mediated plant transformation
- Leaf disc preparation
- Co-cultivation
- Selection media transfer
- Analysis of transgenic plants through PCR for confirming the presence of gene
- Plant Genomic DNA extraction
- Polymerase Chain Reaction
Probable Duration : 3 - 4 Weeks*
Fee Amount : Rs. 9000
* It depends on Individual student’s capacity to learn.
COURSE 3
PLANT TISSUE CULTURE
- Plant tissue culture media preparation and autoclaving
- Cleaning and sterilization procedures
- Isolation of embryos from the seeds and initiation
- Sub-culturing
- Synthetic Seed Preparation
- Agrobacterium mediated plant transformation
- Leaf disc preparation
- Co-cultivation
- Selection media transfer
Probable Duration:8-10 Days*
Fee Amount:Rs. 4,000
* Option :- Plant Genomic DNA Extraction with PCR Rs. 4,750
COURSE 4
IMMUNOLOGICAL STUDIES
- Extraction of total Proteins from different sources.
- SDS-PAGE for running protein samples from different sources including puified virus.
- Western blotting for immuno detection test.
- ELISA for testing the samples for viral (ToMV) infectoion.
Probable Duration :8-10 Days
Fee Amount:Rs.4000
COURSE 5
PROJECT FOR M.Phil., M.Sc., B.E., B.Tech & OTHER STUDENTS
With a Probable duration of 1.5 to 6 Months and
fee Amount Rs. 13,000.00 to 25,000.00
- Production and screening of transgenic plants with particular gene of interest (8 different topics are available)
- Regeneration system Standardization of different Medicinal Plants.(5 Different topics avilable)
- Varietal Identification through DNA Finger Printing - (a) Chilli (b) Tobacco
- Purification & Characterization of Mosaic Virus (ToMV)
- Purification of Enzyme- (a) Taq DNA Polymerase (b) Amylase
- Identification of Male,Female and Hybrid in a given seed lot.(Hybrid seeds purity test)
- Biodegradation of organic matirial by micro organism - (a) Benzene (b) Tolune.
- Commercial Plant Tissue culture - Initiation & multiplication of different crops
Note:
- Whether it is research project or training project, We will guide you but you have to handle everything to get ‘hands on’ experience
- For practicals, each batch will have a maximum of six students
- For outstation students bus facility for pick up and drop from Bangalore station can be arranged at concessional rate
- Comfortable and well manitained accommodation with food avilable [Saparate for boys and Girls],within walking distance from the Research Lab.
- Reaction areas (lake,market,garden) within walking distance from the hostel.
Duration of 1.5 to 6 months
Fee Amount : Rs.13,000 to Rs.25,000